LOCUS       EF1a-FLuc-IRES-Bsd      6807 bp    DNA     linear        8-JUL-2013
SOURCE      
  ORGANISM  
COMMENT     
FEATURES             Location/Qualifiers
     LTR             6573..6807
                     /label=3'\LTR
     intron          291..1455
                     /label=Intron
     LTR             1..181
                     /label=5'LTR
     misc_feature    5894..6490
                     /label=WPRE
     promoter        1698..3087
                     /label=human\EF1a\promoter
     CDS             5486..5884
                     /label=Bsd
     RBS             4878..5462
                     /label=IRES
     CDS             3217..4869
                     /label=Firefly\Luciferase\(FLuc)
     intron          2126..3068
                     /label=intron
BASE COUNT     1622 a      1769 c      1894 g      1522 t 
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga 
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga 
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga 
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa 
     1681 ttcaaaattt tatcgatgcc tccccgtcac cacccccccc aacccgcccc gaccggagct 
     1741 gagagtaatt catacaaaag gactcgcccc tgccttgggg aatcccaggg accgtcgtta 
     1801 aactcccact aacgtagaac ccagagatcg ctgcgttccc gccccctcac ccgcccgctc 
     1861 tcgtcatcac tgaggtggag aagagcatgc gtgaggctcc ggtgcccgtc agtgggcaga 
     1921 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc 
     1981 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt 
     2041 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg 
     2101 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct 
     2161 ctttacgggt tatggccctt gcgtgccttg aattacttcc acgcccctgg ctgcagtacg 
     2221 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt 
     2281 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg 
     2341 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt 
     2401 aaaatttttg atgatatcct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg 
     2461 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg 
     2521 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac 
     2581 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg 
     2641 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc 
     2701 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg 
     2761 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg 
     2821 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta 
     2881 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     2941 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3001 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3061 catttcaggt gtcgtgaaaa ctacccctct agataatacg actcactata gggcccgggt 
     3121 tggatctggc ctcggcggcc aagcttagac actagagggt atataatgga agctcgactt 
     3181 ccagcttggc aatccggtac tgttggtaaa gccaccatgg aagatgccaa aaacattaag 
     3241 aagggcccag cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa 
     3301 gccatgaagc gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag 
     3361 gtggacatta cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag 
     3421 cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc 
     3481 ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc 
     3541 tacaacgagc gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg 
     3601 agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag 
     3661 atcatcatca tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg 
     3721 acttcccatt tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg 
     3781 gacaaaacca tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta 
     3841 gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc 
     3901 aaccagatca tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc 
     3961 atgttcacca cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc 
     4021 gaggaggagc tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg 
     4081 cccacactat ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac 
     4141 ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc 
     4201 aaacgcttcc acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc 
     4261 attctgatca cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc 
     4321 ttcgaggcta aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc 
     4381 gagctgtgcg tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca 
     4441 aacgctctca tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag 
     4501 gacgagcact tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag 
     4561 gtagccccag ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg 
     4621 gtcgccggcc tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa 
     4681 cacggtaaaa ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc 
     4741 gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc 
     4801 aagttggacg cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc 
     4861 gccgtgtaat aattctagcc cctctccctc ccccccccct aacgttactg gccgaagccg 
     4921 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt 
     4981 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct 
     5041 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct 
     5101 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc 
     5161 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc 
     5221 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc 
     5281 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc 
     5341 tgatctgggg cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct 
     5401 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca 
     5461 cactagagat ccaccggtcg ccaccatggc caagcctttg tctcaagaag aatccaccct 
     5521 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc 
     5581 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac 
     5641 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa 
     5701 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg 
     5761 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga 
     5821 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg 
     5881 ctaagcggcc gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 
     5941 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 
     6001 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 
     6061 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 
     6121 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 
     6181 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 
     6241 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 
     6301 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 
     6361 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 
     6421 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 
     6481 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag 
     6541 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 
     6601 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 
     6661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 
     6721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 
     6781 tttagtcagt gtggaaaatc tctagca 
//
